SAMPLE PREPARATION

DNA Isolation

For larger samples (visible pellet):

• Ultra Clean Microbial DNA Isolation Kit (Cat. # 12224) from MO BIO Laboratories, Inc. using a bead-beating step.

For smaller samples (minimal pellet):

• Modified Protocol uses Ready-Lyse™ Lysozyme Solution (Epicentre, Cat. No. R1802M) and MasterPure DNA Purification Kit (Epicentre, Cat. No. MCD85201)
  

Sample Submission

200 ng at a concentration of at least 10 ng/µl of each genomic DNA sample in a 0.5 ml Eppendorf tube must be submitted. Samples must have a 260/280 ratio of 1.6-1.8. DNA samples in solution should be frozen at -80°C or lyophilized prior to shipment. Lyophilization of samples is recommended for international clients.

All frozen samples must be shipped overnight on dry ice.
Lyophilized samples can be shipped at room temperature.

All samples must be clearly labeled without patient identifiers.
Samples will be initially screened for suitability of processing. (see pricing)

Once approved by MIM, ship samples to:

Susan Boches (HOMIM)
The Forsyth Institute
140 The Fenway
Boston, MA 02115