SAMPLE PREPARATION
DNA is isolated from clinical samples by the client and the DNA samples are then submitted. Thus, do not send actual clinical samples unless special arrangements are made in advance. For technicial assistance, contact Sean Cotton at scotton@forsyth.org or (617) 892-8452.
Recommended DNA isolation protocols
For smaller samples (minimal pellet):
- Modified Protocol uses Ready-Lyse™ Lysozyme Solution (Epicentre, Cat. No. R1802M) for overnight incubation and MasterPure DNA Purification Kit (Epicentre, Cat. No. MCD85201)
For larger samples (visible pellet):
- Ultra Clean Microbial DNA Isolation Kit (Cat. # 12224) from MO BIO Laboratories, Inc. using a bead-beating step.
At least 200 ng at a concentration of a minimum of 10 ng/µl of each genomic DNA sample in a 0.5 ml or 1.5 ml Eppendorf tube must be submitted. However, samples at higher concentrations are preferred. Samples must have a 260/280 ratio of 1.6-2.0. DNA samples in solution should be frozen at -80°C. Lyophilization of samples is recommended for international clients.
All frozen samples must be shipped overnight on dry ice (note there are no weekend or holiday deliveries to the Forsyth Institute).
Lyophilized samples can be shipped at room temperature.
All samples must be clearly labeled without patient identifiers.
Samples will be initially screened for suitability of HOMIM processing (see pricing).
Once approved by MIM, ship samples to:
Sean Cotton (HOMIM)
The Forsyth Institute
140 The Fenway
Boston, MA 02115