BACKGROUND
HOMIM was developed as a high throughput technology to examine complex oral microbial diversity in a single hybridization, i.e., simultaneous detection of about 300 predominant oral bacterial species, including the “uncultivables”. HOMIMs are used extensively in ongoing studies at The Forsyth Institute (6 PIs) and in other collaborative studies with national and international academic institutions (30 investigators or teams), government including NIH (6 investigative teams), and industry (8 small and large companies). As a result of some of these studies, there are already 3 peer-reviewed publications, 3 reviews describing HOMIM, and many abstracts as listed below.
Background and HOMIM-associated publications
Paster BJ, Dewhirst FE. Molecular microbial diagnosis. Periodontol 2000 2009;51:38-44.
Colombo et al. Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis and periodontal health using the human oral microbe identification microarray. J Periodontol 2009;80:1421-1432.
Olsen el al. Cultivated and not-yet-cultivated bacteria in oral biofilms. Microb Ecol Hlth Dis 2009;21:65-71.
Preza et al. Diversity and site-specificity of the oral microflora in the elderly. Eur J Clin Microbiol Infect Dis 2009; 28:1033-1040.
Preza et al. Microarray analysis of the microflora of root caries in elderly. Eur J Clin Microbiol Infect Dis 2008;28:509-517.
Paster et al. The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol 2000 2006;42:80-87.
Aas et al. Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 2005;43:5721-5732.
Paster et al. Bacterial diversity in human subgingival plaque. J Bacteriol 2001;183:3770-3783.
Colombo et al. Subgingival microbiota of refractory periodontitis determined by microarray (HOMIM). J Dent Res 2009; 88: #232. See publication in J Periodontol
Albandar et al. Molecular analysis of Papillon-Lefèvre Syndrome periodontitis subgingival microbiota. J Dent Res 2009; 88: #1373.
Cotton et al. Subgingival taxa in periodontal health and disease using HOMIM. J Dent Res 2009; 88: #1378.
Yaskell et al. Comparison of checkerboard and HOMIM for detecting subgingival species. J Dent Res 2009; 88: #1379.
Preza et al. Microarray analysis of bacteria in root caries. J Dent Res 2009; 88: #3086.
Zhang et al. Multiple salivary biomarkers for pancreatic cancer detection. J Dent Res 2009; 88: #3399. Submitted for publication
Kanasi et al. Microarray analysis of the microbiota of young children. J Dent Res 2008; 87: #3418.
Wong et al. Salivary biomarkers for pancreatic cancer detection. Am. Soc. Clin. Oncol. May 29-June 2, 2009, Orlando, FL.
Uraz et al. Microbial microarray analysis of gingival crevicular fluid in periodontitis. Europerio 6; 6th Congress of the European Federation of Periodontology, June 4-6, 2009, Stockholm, Sweden.